Detection of p53 gene mutations in human gestational trophoblastic neoplasia by polymerase chain reaction-single strand conformational polymorphism techniques

Wu, Y.; Zhang, Q.; Tang, H.; Wu, Y; Zhang, Q; Tang, H

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Detection of p53 gene mutations in human gestational trophoblastic neoplasia by polymerase chain reaction-single strand conformational polymorphism techniques

Wu, Y.; Zhang, Q.; Tang, H.

Hunan Yi Ke Da Xue Xue Bao 22(4): 338-340

1997

ISSN/ISBN: 1000-5625

PMID: 9868092

Document Number: 471229

Using polymerase chain reaction single strand conformational polymorphism (PCR-SSCP) and EB staining technique, paraffin-embeded sections of 20 hydatidiform mole and 4 choriocarcinoma were detected in the exons 5 and 8 of p53 gene. The results showed that mutations of p53 gene were 0/9 in the normal chorionic villi, 6/20 (30%) in hydatidiform mole and 3/4 in choriocarcinoma. This study suggests that mutations of p53 gene may be an important event in human gestational trophoblastic neoplastigenesis and its progression.