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Preparation of novel macroporous silica-based amide-polymer-bonded packing and its application to the separation of proteins

Wei, Y.; Fan, L.; Jiang, S.; Chen, L.

Se Pu 16(3): 191-194

1998

ISSN/ISBN: 1000-8713
PMID: 11326995
Document Number: 496757
A novel macroporous silica-based amide-polymer-bonded packing for protein separations in HPLC is described. The macroporous silica support was bonded with diethoxymethyl vinyl silane and then copolymerized with methylacrylamide and divinylbenzene to produce a tailored stationary phase with high resolution and inertness. The repeatability of packing preparation is good. They were characterized through the application of a standard protein mixture of pepsin, glucose oxidase, bean trypsinogen inhibitor and ribonuclease. The time for elution of these proteins is less than 12 minutes. It is suggested that the macroporous silica-based amide-polymer-bonded packing can be used to quickly separate biopolymer. This packing is a better alternative for the biopolymer separation.

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Wheals, B.B. 1979: Simple preparation of a bonded cation-exchange packing material and its application to the separation of phenothiazines by high-performance liquid chromatography Journal of Chromatography 177(2): 263-270
Jones, G. 1983: Chromatographic separation of 24(R),25-dihydroxyvitamin D3 and 25-hydroxyvitamin D3-26,23-lactone using a cyano-bonded phase packing Journal of Chromatography 276(1): 69-75
Xu, L.; Li, L.; Yang, H. 2007: Preparation, characterization and chromatographic performance of curcumin bonded silica stationary phase Se Pu 25(3): 374-379
Yang, R.; Jiang, S.; Liu, X.; Chen, L. 1999: Preparation and application of a new chloromethylstyrene encapsulated silica single anion column Se Pu 17(3): 296-298
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Filatova, T.N.; Gamzulina, L.N.; Zamiatina, T.A. 1995: The use of silica gel-based ion-exchange sorbents for isolating one of the iron-dependent proteins of meningococci Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 6: 19-20
Yan, L.; Zhang, Q.; Li, T.; Zhang, W.; Feng, Y.; Zhang, L.; Zhang, Y. 2005: Preparation and evaluation of organic-inorganic hybrid silica-based monolithic columns for capillary electrochromatography Se Pu 23(5): 499-503
Xu, H.-L.; Chen, J.; Shao, J.-Z.; Sun, Y.; Zhang, C.-Z. 2006: Study on separation and purification of anthraquinones in radix et rhizoma rhei by D301 macroporous resin Zhongguo Zhong Yao Za Zhi 31(14): 1163-1165
Wang, G.-s.; Hou, S.-x.; Zhu, H.; Mao, S.-j.; Bi, Y.-q. 2006: Studies on characteristics of absorption and separation of traditional Chinese medicine compound prescription by macroporous resin Zhongguo Zhong Yao Za Zhi 31(15): 1237-1240
Xu, Y.-W.; Qi, Y.; Han, X.; Xu, L.-N.; Xu, Q.-W.; Peng, J.-Y. 2007: Preparative separation of gentiopicrin from Radix Gentianae by high-speed counter-current chromatography with macroporous resin Zhongguo Zhong Yao Za Zhi 32(24): 2595-2597
Zhou, R.; Feng, Y.-q.; Da, S.-l. 2002: Studies on the retention mechanism of N-methylaniline bonded silica for high performance liquid chromatography Se Pu 20(3): 193-196
Flurer, C.L.; Borra, C.; Andreolini, F.; Novotny, M. 1988: Separation of proteins by microcolumn liquid chromatography based on the reversed-phase and size-exclusion principles Journal of Chromatography 448(1): 73-86
Schilling, K.; Horn, A.; Börnig, H. 1972: A preparative membrane electrophoresis apparatus based on the barrier principle for the separation of proteins in gram quantities Acta Biologica et Medica Germanica 29(3): 341-348
Mesplet, N.; Morin, P.; François, C.; Agrofoglio, L.A. 2001: Simultaneous quantitation of nucleoside HIV-1 reverse transcriptase inhibitors by short-end injection capillary electrochromatography on a beta-cyclodextrin-bonded silica stationary phase Journal of Chromatography A 927(1-2): 161-168
Ishimura, K.; Fukunaga, K.; Irie, T.; Uekama, K.; Ohta, T.; Nakamura, H. 1997: Application of a beta-cyclodextrin sulfate-immobilized precolumn to selective on-line enrichment and separation of heparin-binding proteins by column-switching high-performance liquid chromatography Journal of Chromatography A 769(2): 209-214
Kanayama, M.; Kawamoto, R.; Suzuno, N.; Hayashi, M.; Sakamoto, K. 1988: Preparation and application of the nursing standard based on the nursing process. 8. Preparation of the standard for the problems common among patients following rectum resection--the problem of urination disorders Nursing Technique 34(11): 1363-1371
Emirbekov, E.Z.; Musaev, B.S. 1971: Amide groups of gopher brain proteins in hypothermia Ukrains'kyi Biokhimichnyi Zhurnal 43(6): 697-700
Papian, A.A. 1974: Amide groups of brain proteins during dying and resuscitation of the body Zhurnal Eksperimental'noi i Klinicheskoi Meditsiny 14(5): 28-31
Meng, Z.; Wang, J.; Zhou, L.; Wang, Q.; Zhu, D. 1999: Beaded molecule imprinted polymer for stereo isomer separation Se Pu 17(4): 323-325
Kochagin, V.B.; Serova, L.I.; Demet'eva, S.P.; Novol'neva, I.N.; Inozemtseva, I.I. 1971: Thin layer silica gel chromatography of penicillins. Separation of phenoxymethylpenicillin in its inactivation products Antibiotiki 16(1): 8-11