THe use of a biotin-labelled DNA probe for detecting the causative agents of chronic urogenital infections
Kvasov, A.V.; Zhdanov, A.V.; Pis'menskaia, I.V.; Faĭzullina, N.M.; Faĭzullin, L.Z.; Sukhikh, G.T.
Klinicheskaia Laboratornaia Diagnostika 11: 12-15
1998
ISSN/ISBN: 0869-2084 PMID: 10205651 Document Number: 495591
DNA hybridization for detecting HSV, CMV, C. trachomatis, and U. urealyticum by biotin-labeled DNA probe was used for investigating clinical specimens from patients with infertility and chronic urogenital inflammations. High sensitivity and specificity of the method was confirmed by the results of PCR, ELISA, and immunofluorescent methods in 80-90% cases. DNA hybridization technique is a simple method requiring no sophisticated equipment, which recommends it for the diagnosis of sexually-transmitted diseases at clinical laboratories.
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