A method of isolating the main outer membrane protein of Chlamydia
Bilozorov, O.P.
Mikrobiolohichnyi Zhurnal 60(3): 85-90
1998
ISSN/ISBN: 0201-8462 PMID: 9785804 Document Number: 495417
Purified chlamydial bodies were solubilized by detergent solutions used in the following sequence: 1) 1% sarcosil, 2) 1% sarcosil + 10 mM dithiotreitol, 3) 2% sodium dodecyl sulfate + 10 mM dithiotreitol. After the third stage a good yield of protein, corresponding to major outer membrane protein as to its molecular weight and antigenic properties was obtained.
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