Effect of pilocarpine in tissue structures of the anterior eye segment from the aspect of its interaction with free amino acids
Veselovský, J.; Oláh, Z.; Veselovská, Z.; Veselá, A.
Ceska a Slovenska Oftalmologie Casopis Ceske Oftalmologicke Spolecnosti a Slovenske Oftalmologicke Spolecnosti 54(1): 10-17
1998
ISSN/ISBN: 1211-9059 PMID: 9501638 Document Number: 493478
On the basis of the proofs of pilocarpine interaction with free amino acids, the mechanism of this alkaloid effect in the tissue structures of the eye anterior segment is explained. Differences of colour reactions of amino acids mixtures with pilocarpine, decrease of radioactivity of Na[I125]tyrosine mixture with pilocarpine, unsteadiness of radioactivity of Na[I125]protein A mixture with pilocarpine and rise of 16 radioactivity fractions after adding pilocarpine to Na[I125]proteins A a) show the specificity of interaction of each amino acid with pilocarpine; b) give evidence of a strong covalent bond between pilocarpine and free amino acid and give rise to new biologically active metabolite; c) show non-cholinergic pathway of pilocarpine effect; d) for ophthalmo-physiological practice it is said that with respect to the rate between pilocarpine quantity and concentration and free amino acid quantity and concentration requested therapeutical effect by lower pilocarpine dose in glaucoma disease treatment might be achieved. Thus free amino acids being reactants can be identified as mechanism by which pilocarpine becomes biologically active.