Effect of ganglioside GM1 on the metabolism of Alzheimer amyloid beta-protein precursor

Zhang, D.; Ruan, Y.; Beyreuther, K.

Zhonghua Yi Xue Za Zhi 78(3): 179-182


ISSN/ISBN: 0376-2491
PMID: 10923524
Document Number: 493103
To study the effect of GM1 ganglioside interfering the metabolism of amyloid beta-protein precursor (APP) on cells for exploring the relationship between GM1 and development of Alzheimer senile plaques and the other amyloidoses. GM1 influencing the metabolism of APP and the release of its metabolite secretory APP on human APP695 cDNA transfected cells were detected by metabolic radiolabeling. The affinity of GM1 and recombinant secretory APP was analyzed by Western blot and dot blot. GM1 specifically bound APP, and interfered the proteolysis of APP and inhibited the release of secretory APP on cells. The abnormal metabolism of GM1 may contribute to the aggregation and deposition of amyloid beta-protein in Alzheimer brains.

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