Karyotypic instability of peripheral blood lymphocytes in cows Bos taurus L. infected with bovine leukemia virus
Dubik, E.P.; Treus, V.V.; Nikitin, N.S.; Smirnov, A.F.
Tsitologiia 40(4): 317-322
1998
ISSN/ISBN: 0041-3771 PMID: 9644765 Document Number: 491484
Chromosomal aberrations (CAs), sister-chromatid exchanges (SCEs), aneuploidy and proliferative potential (PP) were investigated in peripheral blood lymphocytes of healthy cows (control group-C), BLV-(bovine leucosis virus)-infected cattle without hematological abnormalities (RID--seropositive group (I) and affected with leucaemia (lymphocytosis (LC), lymphoma (L)). Nonrandom chromosomal (marker) aberrations were not found in the cow group at stage LC. The levels of aneuploidy and SCEs increased in the cow group at stage L compared to the cow group at stage I. Polyploidy: C--1.9 +/- 0.28, I--3.5 +/- 0.22, LC--6.1 +/- 0.82, L--10.5 +/- 0.51 (P < 0.01). Hypoploidy (2n = 58): C--3.0 +/- 0.17, I--54 +/- 0.71, LC--12.1 +/- 0.72, L--14.0 +/- 0.65 (P < 0.01). SCEs: C--3.8 +/- 0.26, I--5.4 +/- 0.15, LC--7.2 +/- 0.16, L--9.7 +/- 0.26 (P < 0.01). There are no differences in CAs rates and PP between groups of cows at all the observed stages of leucaemic process. The obtained results are discussed in terms of cytogenetic aspects of leucaemic process in cows.