Early diagnosis of nasopharyngeal carcinoma using recombinant antigens expressed in bacteria
Wan, Z.; Pi, G.; Sun, N.
Zhonghua Shi Yan he Lin Chuang Bing du Xue Za Zhi 12(1): 74-76
1998
ISSN/ISBN: 1003-9279 PMID: 12515179 Document Number: 487951
An enzyme-linked immunosorbent assay(ELISA) was developed by using recombinant purified early antigens, EA-D and EA-R to detect IgA antibodies in sera from nasopharyngeal carcinoma(NPC) patients. 30 sera from NPC patients and 49 sera from normal persons were detected by the developed ELISA. The serum positive rate and antibody titer were compared with immunoenzymatic method(IE) on slide with Raji cells mears. ELISA is more sensitive and specific. The serum positive rate of NPC patients was increased from 70% by IE to 100% by ELISA and 77% of the serum antibody titer reached > or = 1:200. The results show that the expressed recombinant p138 and p54 antigens are effective for detection of EA/IgA antibody in sera from NPC patients and ELISA will be a sensitive, specific and convenient method for early diagnosis of NPC.