Preparation and properties of pancreatin immobilized on carboxymethylcellulose
Nekliudov, A.D.; Ivankin, A.N.; Baburina, M.I.
Prikladnaia Biokhimiia i Mikrobiologiia 34(1): 61-65
1998
ISSN/ISBN: 0555-1099 PMID: 9566291 Document Number: 486607
A method for immobilizing pancreation on carboxymethylcellulose (CMC) is proposed. The optimum reaction conditions were determined: CMC, 1 x 10(-4) M; 1-ethyl 3-(dimethylaminopropyl)-carbodiimide, 2 x 10(-4) M; incubation time, 18 h at 4 degrees C. They allow obtaining a conjugate of the enzyme with the polymer displaying 85% of the protease and 92% of the esterase activity. Both the native and immobilized enzymes were shown to contain thermolabile and thermostable fractions with different inactivation constants. Immobilization of the enzyme was found to increase its thermal stability by a factor of 1.5 to 3. Thermodynamic constants of blood protein hydrolysis by native and immobilized enzymes were determined.