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Construction of PCNA antisense RNA expression vector and its antitumorigenic effect on human gastric cancer cell

Dai, L.; Zhang, X.; Hui, H.

Zhonghua Zhong Liu Za Zhi 20(5): 337-339

1998

ISSN/ISBN: 0253-3766
PMID: 10921024
Document Number: 486371
To observe the effect of proliferating cell nuclear antigen (PCNA) antisense RNA on the growth characteristics of human gastric cancer cell. An eukaryotic expression vector pDR-PCNA with human PCNA gene insert in reverse direction was successfully constructed. The human gastric cancer cell line SGC-7901 was transfected with pDR-PCNA by lipofectamine. Growth rate, protein and RNA biosynthesis and DNA contents in S-phase and G2/M-phase of the transfected SGC-7901 were significantly suppressed in comparison with those of the parental cell line. PCNA antisense RNA can significantly suppress the in vitro growth of gastric cancer cell line.

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