Extraction and characterization of native canine bone morphogenetic protein (cBMP) qualified with osteoinductivity
Oksanen, J.; Marttinen, A.; Paatsama, S.; Lindholm, T.S.
Acta Veterinaria Scandinavica 39(2): 165-171
1998
ISSN/ISBN: 0044-605X PMID: 9787480 Document Number: 485440
Partially purified bone morphogenetic protein (BMP) extracted from canine bone matrix was tested for osteoinductivity. A radiographically and histologically detectable ectopic bone formation was induced by 6.0 mg canine (cBMP) in muscle pouch of BALB mouse at 21 days after implantation. Characterization of the cBMP preparation by a gel filtration chromatography defined that the material consisted of proteins or protein complexes with molecular weights from 4 to 120 kDa. Isoelectric focusing showed that the molecules were acidic with isoelectric points of 4.6-5.6.