Effect of U-73122 on calcium channel activity in porcine myometrial cells and pancreatic beta-cell line RINm5F
Li, S.B.; Chen, T.H.; Zhu-Ge, R.H.; Hsu, W.H.
Zhongguo Yao Li Xue Bao 18(5): 385-390
1997
ISSN/ISBN: 0253-9756 PMID: 10322922 Document Number: 483495
To study the effect of U-73122, putative phospholipase C (PLC) inhibitor, on cytosolic Ca2+ concentration ([Ca2+]i) and voltage-dependent calcium channels (VDCC). To record whole cell calcium current (ICa) by perforated patch-clamp and to measure cytosolic free calcium concentration ([Ca2+]i) with Fura-2. U-73122 inhibited KCl-induced and Bay-K-8644-induced increase in [Ca2+]i dose-dependently. U-73122 decreased markedly depolarization-induced ICa both in RINm5F and porcine myometrial cells in a concentration-dependent manner. The effect of U-73122 was more potent in porcine myometrial cells than that in RINm5F cells. U-73343 (an analog of U-73122), that does not inhibit PLC, also reduced the ICa by 50%. U-73122 inhibited VDCC and [Ca2+]i in both RINm5F and porcine myometrial cells.