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Determination of HbA1c in the presence of haemoglobin variants: comparison of three HPLC techniques

Carta, M.; Dall'Olio, G.; Soffiati, G.

European Journal of Clinical Chemistry and Clinical Biochemistry 35(12): 923-925

1997

ISSN/ISBN: 0939-4974
PMID: 9476621
Document Number: 482874
The presence of Hb variants may cause analytical interference in HbA1c values measured with the HPLC techniques currently used in Italian laboratories. In this study performance of a new HPLC system, (HA-8140, Menarini) was compared with two other traditional HPLC systems (HA-8121, Menarini and Diamat, Bio-Rad). The HA-8140 system detects the Hb variant possibly present in an independent peak. The integration area of such a peak is not computed in the calculation of the percentage value of HbA1c. In this manner the underestimation of values obtained with traditional HPLC systems is avoided.

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