Approaches to selecting a strain of hantavirus for developing an inactivated brain vaccine
Astakhova, T.I.; Slonova, R.A.; Kompanets, G.G.
Voprosy Virusologii 42(3): 105-108
1997
ISSN/ISBN: 0507-4088 PMID: 9297337 Document Number: 481018
Four strains isolated from patients with hemorrhagic fever with the renal syndrome and from field mice, natural hosts of Hantavirus, were examined in order to choose a functionally active strain fit for the development of inactivated Hantavirus brain vaccine. Virulent strain PM-10508/89 isolated from mouse lungs was chosen. The strain was highly virulent for newborn mice and rats: 100% of infected mice aged up to 24 h and 75% of rats developed an acute lethal infection upon challenge. The incubation period of Hantavirus adapted to mouse brain was decreased from 19 to 7 days. The infective titer of the virus in brain suspension was 0.1 to 6.5 lg LD50/ml. The strain was highly immunogenic and possessed a high hemagglutinating activity.