Bactericidal activity determination of Biseptine, combination of chlorhexidine, benzalkonium chloride and benzylic alcohol, on 124 hospital bacterial strains
Reverdy, M.E.; Martra, A.; Fleurette, J.
Pathologie-Biologie 45(4): 331-335
1997
ISSN/ISBN: 0369-8114 PMID: 9296082 Document Number: 479992
Biseptine is an antiseptic composed of chlorhexidine digluconate (CHX), benzalkonium chloride (BC) and benzylic alcohol. Minimal Bactericidal Concentrations (MBCs) of Biseptine were determined on 124 clinical strains: 76 Enterobacteriaceae, 16 other Gram negative bacilli, (Pseudomonas spp, Aeromonas spp, Haemophilus spp) and 32 Gram positive bacteria (Staphylococcus spp, Streptococcus spp, Listeria spp, Bacillus cereus), using microdilution method, in comparison with Hibitane Champ. Modal MBC of Biseptine was 25 mg/l of CHX/2.5 mg/l BC (1/100 dilution). Proteus (MBC: 133 mg/l CHX/ 13 mg/l CB) and B. cepacia (MBC: 100 mg/l CHX/ 10 mg/l CB) were the most resistant strains, as expected with cationic antiseptics. 4/5 Bacillus cereus, strains were weakly susceptible to Biseptine and Hibitane Champ. In Biseptine, the association of CHX and CB showed a synergic activity, MBCs are usually 2 fold lower that Hibitane Champ MBCs.