In-vitro effect of estrogen-antagonist on motility and penetration ability of human spermatozoa
Allag, I.S.; Rangari, K.
Indian Journal of Experimental Biology 35(8): 822-824
1997
ISSN/ISBN: 0019-5189 PMID: 9475054 Document Number: 477824
Antiestrogens affect spermatozoa through their action on Leydig and Sertoli cells. Direct effect of antiestrogens namely tamoxifen and centchroman in concentration of 1, 2.5, 5, 10 and 20 micrograms/ml in incubation medium was determined on motility and penetration ability of human spermatozoa. Motility (%) was invariably reduced after 15, 30 and 60 min. of incubation. Addition of 17 beta-estradiol to medium with antagonist caused inhibition of motility in dose related manner. The distance travelled by spermatozoa treated with tamoxifen or centchroman in media was reduced by 30% and addition of estradiol along with antiestrogen reduced it to 50% compared to that of untreated spermatozoa.