Effects of estradiol and its antagonist tamoxifen on motility and metabolism of human spermatozoa
Idaomar, M.; Guerin, J.F.; Lornage, J.; Moncharmont, P.; Czyba, J.C.
Advances in Contraceptive Delivery Systems Cds 3(4): 337-345
1987
ISSN/ISBN: 1012-8689 PMID: 12341905 Document Number: 294644
An investigation was conducted to evaluate the motility and metabolism of human spermatozoa incubated at 37.degree.C for 3 hours in a krebs phosphate Ringer solution containing different concentrations of 17.beta. estradiol and tamoxifen. 17.beta. estradiol (17.beta. E2) stimulated significantly the motility of human spermatozoa. The oxidative metabolism and the intracellular ATP concentrations are enhanced with 10 20 .mu.g/ml of 17.beta. E2, the lactate production remains however, unchanged. Tamoxifen, an antiestrogen, inhibited the motility of spermatozoa, and was toxic at high concentrations. Its effects on the energetic metabolism is dose dependant: it increases the oxidative metabolism and decreases the lactate production and intracellular ATP at 3 .mu.g/ml.