Insertion mutations in Pseudomonas pseudomallei genome induced by transposons Tn10. Tn9, and Tn5
Merinova, L.K.; Timofeeva, E.V.; Simova, I.K.
Molekuliarnaia Genetika Mikrobiologiia i Virusologiia 1997(1): 14-17
1997
ISSN/ISBN: 0208-0613 PMID: 9082181 Document Number: 475340
Tn10 and Tn9 from temperature-sensitive replicons RP1::Tn10 Rep is and RP1::Tn9 Rep is and Tn5 from the "suicidal" vector pSUP5011 may integrate in the P. pseudomallei chromosome. Transposons induced auxotrophic mutations of different spectrum, depending on the strain, Tn element, and defects in the genes responsible for the mobility sign, function of some extracellular elements (lecithinise and lipase), hemolytic activity, and antigen 8 synthesis, which are considered is the potential factors of the pathogenicity of melioidosis agent. No secondary restructuring induced by Tn elements were detected in the domains adjacent to insertion sites. Plasmids RP1::Tn10 Rep ts and RP1::Tn9 Rep ts were stable in insertion mutant cells, mediating the variable T-R-T-S phenotype probably indicating that plasmid integration with tile chromosome is unstable.