The immunogenicity and heterogeneity of Pseudomonas pseudomallei surface antigen 8
Piven', N.N.; Smirnova, V.I.; Viktorov, D.V.; Kovalenko, A.A.; Farber, S.M.; Iarulin, R.G.; Podzolkova, G.G.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 4: 75-78
1996
ISSN/ISBN: 0372-9311 PMID: 9027183 Document Number: 467433
Surface antigen 8, one of pathogenicity factors of P.pseudomallei and having an antiphagocytic action, contains glycoprotein with a mol. wt. of 200 kD and proteins with mol wt. of 25, 30 and 34 kD. According to its chemical structure, the carbohydrate part of antigen 8 is the homogeneous polymer of 6-d-D-mannoheptose, and its protein component is a collection of monomer proteins with mol. wt. of 12-120 kD. The consecutive fractionation of antigen 8 by gel and ion exchange chromatography has made it possible to isolate its individual fragments, differing by the collection of antigenic components, as well as by their antiphagocytic and protective activity. Experiments have shown that glycoprotein with a mol. wt. of 200 kD is an active immunosuppressive agent, while protein with a mol. wt. of 34 kD produces a pronounced immunogenic effect.