Identification of Eucalyptus citriodora clones micropropagated in tissue culture
Kojima, E.; Izumi, M.; Tanabe, T.; Matsuda, M.; Shimizu, T.; Murakami, A.; Murakami, K.
Nucleic Acids Symposium Series 37: 171-172
1997
ISSN/ISBN: 0261-3166 PMID: 9586054 Document Number: 474914
The extent of genetic identity observed in the young individuals which were micropropagated from a single Eucalyptus individual was analyzed by using DNA-fingerprinting. Among 40,000 tissue-cultured-seedings of E.citriodora, 200 plants were randomly chosen so that each total DNA might be extracted from their leaves. Using these DNAs as template, PCR was performed with some primers we found in advance that leads polymorphism for DNA of E. citriodora. In this study, all over the 200 cases, the band pattern formed cDNA fragment on a gel after electrophoresis was the identical one mutually.