DNA sequence dependent binding modes of bis (vinylpyridinium) benzene derivatives
Juskowiak, B.; Takenaka, S.; Takagi, M.; Kondo, H.
Nucleic Acids Symposium Series 37: 265-266
1997
ISSN/ISBN: 0261-3166 PMID: 9586101 Document Number: 473749
The DNA binding selectivity of new dicationic ligands based on the bis(vinylpyridinium)benzene unit has been investigated by means of UV-Vis absorption spectroscopy. From the experimental results it is concluded that these extended pi-electron bridged viologens have relatively high affinity to AT base pair sequences whereas the binding to GC pairs is about 10 times lower, and binding affinity depends on minor variation in the ligand structure. Linear type ligand exhibits two binding mode interaction, intercalation at high dye concentration which undergoes switching to groove binding at low ligand concentration.