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Nonradioactive assay for sequence-specific DNA binding proteins

Gubler, M.L.; Abarzúa, P.

Biotechniques 18(6): 1008 1011-4

1995

ISSN/ISBN: 0736-6205
PMID: 7546700
Document Number: 453485
A nonradioactive functional assay was developed to quantitate DNA binding proteins. The assay was designed to allow the use of 96-well microplates for high sample throughput. We show that the assay can measure sequence-specific DNA binding of purified proteins as well as DNA binding activity present in whole cell extracts. By using a temperature-sensitive DNA binding protein, we demonstrate that DNA binding as measured by this novel assay correlates with the biological function of the protein.

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