-GG- specific cleavage via hole hopping through double-stranded DNA
Nakamura, T.; Dohno, C.; Nakatani, K.; Saito, I.
Nucleic Acids Symposium Series 37: 87-88
1997
ISSN/ISBN: 0261-3166 PMID: 9586012 Document Number: 473448
We examined the photoinduced DNA cleavage reaction of 32P-end labeled oligodeoxyribonucleotides which contain 5'-TGGGC-3', 5'-CGGGC-3', and 5'-TGGGT-3'. As for 5'-TGGGC-3' and 5'-TGGGT-3', the cleavage occurred most strongly at the middle of 5'-GGG-3' step, whereas the strongest cleavage occurred at the 5' side of 5'-GGG-3' in 5'-CGGGC-3' step. These results showed the 5' side base of 5'-GGG-3' step was very important for the sequence selectivity of 5'-GGG-3' cleavage.