Methods for the detection of antibodies to native (double stranded) DNA
Mitrou, P.S.; Drahovsky, D.; Mitrou, G.; Borck, W.
Medizinische Klinik 74(21): 813-819
1979
ISSN/ISBN: 0025-8458 PMID: 314037 Document Number: 144583
Three different methods adequate for routine work, i.e. hemagglutination, counterimmunoelectrophoresis and radioimmunoassay, were used in the past in our laboratory to detect antibodies to native (double stranded) DNA. There was a high incidence of false positive or false negative results by counterimmunoelectrophoresis. Hemagglutination reaction was less sensitive in detecting anti-nDNA than was radioimmunoassay. Natural DNA preparations and a synthetic polynucleotide (3H-dAT) were used as antigenic substrates in radioimmunoassay providing well correlating binding values.