An animal model for screening of antiallergic and antipruritic drugs
Xu, J.H.; He, Y.X.; Shu, S.T.; Wang, L.M.
Yao Xue Xue Bao 31(6): 420-424
1996
ISSN/ISBN: 0513-4870 PMID: 9275721 Document Number: 469104
4-Aminopyridine(4-AP) 1 mg cntdot kg-1 sc at the scruff induced a licking response in mice. Antiallergic and antipruritic drugs, such as diphenhydramine HCl (20 mg cntdot kg-1 ip), doxepin (12.5 mg cntdot kg-1 ig), prednisone (10 mg cntdot kg-1 ig), dexamethasone (10 mg cntdot kg-1 ip), fluocinolone (applied to the surface of skin), disodium cromoglicate (400 mg cntdot kg-1 ip), ketotifen (1 mg cntdot kg-1 ip), etc. markedly inhibited the licking response elicited by 4-AP. The calcium antagonist nifedipine (500 mg cntdot kg-1 ig) and the potassium channel opener minoxidil(400 mg cntdot kg-1 ig) produced the same inhibitory effect. H-2-receptor antagonist cimetidine (200 mg cntdot kg-1 ip) and ranitidine(150 mg cntdot kg-1 ip) showed no effect. Morphine HCl(10 mg cntdot kg-1 ip) and diazepam(0. 02 mg cntdot kg-1 ip) exhibited antagonistic effect on the licking response induced by 4-AP, but phenobarbital(25 mg cntdot kg-1 ip) , pentobarbital(15 mg cntdot kg-1 ip) and aspirin(300 mg cntdot kg-1 ig) did not. These results indicate that many antiallergic or antipruritic drugs inhibited the licking response induced by 4-AP. The method of licking response elicited by 4-AP has the merit of simplicity and convenience and may be used for screening antiallergic and antipruritic drugs.