Effects of antiallergic drugs on superoxide anion generation from activated human alveolar macrophages measured by chemiluminescence method
Kurosawa, M.; Ishizuka, T.; Kobayashi, S.; Nakano, M.
Arzneimittel-Forschung 41(1): 47-51
1991
ISSN/ISBN: 0004-4172 PMID: 1675572 Document Number: 369276
Human alveolar macrophages were recovered by bronchoalveolar lavage and superoxide anion (O2-) generation from the macrophages were measured by 2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazol[1,2-a]pyrazin-3-one (MCLA)-dependent luminescence. Addition of 0.5 .mu.mol/l MCLA and a stimulatory agent, such as phorbol myristate acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP), to a suspension of the cells caused a marked luminescence which was inhibited by 0.5 .mu.mol/l superoxixde dismutase (SOD). The maximal luminescence intensity was dependent upon the number of the cells. Azelastine (A-5610, CAS 5885-89-8) significantly inhibited the O2 generation from the activated macrophages in a dose-dependent manner. However, the other antiallergic drugs, such as ketotifen, disodium cromoglycate and others showed little effects on the O2- generation from the activated human alveolar macrophages.