Direct detection of the antioxidant activity of a new flavonic derivative using the chemiluminescence method
Saramet, A.; Dănilă, G.; Păduraru, I.; Petrariu, D.; Olinescu, R.
Arzneimittel-Forschung 46(5): 501-504
1996
ISSN/ISBN: 0004-4172 PMID: 8737635 Document Number: 468815
The antioxidant potential of a new water soluble flavonic derivative, namely theophylline rutoside (TR-1722) has been tested using the chemiluminescence method. The method is based on the oxidative degradation of luminol by the hydrogen peroxide in Tris-HCl-buffer, when reactive species of oxygen are being obtained: O-2- cntdot , HO cntdot , 1O-2, and allows for the capability of substances to inhibit the free radical processes in this test system to be quantified and hence for their antioxidant properties in respect of a standard substance (in our case quercetin) to be compared. The results obtained reveal that TR-1722 has antioxidant action comparable to that of quercetin, the highest efficacity being registered at the concentration of 2 mu-mol/l, the conditions being: H-2O-2 16,2 mmol/l; luminol 2 mu-mol/l, in Tris-HCl buffer 20 mmol/l, pH 8.3. The antioxidant potential of TR-1722 is also maintained when the conditions of the system are modified, that is, the concentration of the hydrogen peroxide, the intensity of the action being dependent on the hydrogen peroxide concentration, but no direct proportionality is registered.