Top Abstract Purchase Related
Home
>
Node
>
Subnode

Assessment of immunogenicity and viability of homologous human cardiac valves in vitro

Ketheesan, N.; Kearney, J.N.; Ingham, E.

Journal of Heart Valve Disease 5(2): 144-147

1996

ISSN/ISBN: 0966-8519
PMID: 8665005
Document Number: 468111
Degenerative changes in a proportion of implanted homologous cardiac valves are considered to be due to the immunologic tissue reactions initiated by the donor valves. Sterilization and storage protocols can be used to modulate the immunogenicity of donor valves prior to implantation. Therefore, it is essential to assess the effect of treatment protocols on the immunogenicity and viability of donor valve tissue. The optimal conditions for two novel in vitro tests to assess the immunogenicity and viability of human cardiac valve tissue, the valve cusp cell/responder lymphocyte reaction to assess immunogenicity and the tetrazolium based colorimetric assay to assess viability of valve tissue, are described. The in vitro tests that have been developed in this study will be helpful in assessing the effect of various treatment protocols on the immunogenicity and viability of homologous human cardiac valves.

Document emailed within 1 workday
Secure & encrypted payments

Nuno-Conceiçrao, A.; Puig, L.B.; Verginelli, G.; Iryia, K.; Bittencourt, D.; Zerbini, E.J. 1975: Homologous dura mater cardiac valves. Structural aspects of eight implanted valves Journal of Thoracic and Cardiovascular Surgery 70(3): 499-508
Yu, J.-H.; Guo, H.-W.; Liu, M.-M.; Zhang, G.; Wu, S.-M. 2009: Comparative study on the immunogenicity of human homologous cardiac valve and great artery homograft cryopreserved with liquid nitrogen Zhonghua Yi Xue Za Zhi 89(31): 2225-2228
Martin, A.J.; Christy, J.R. 2004: An in-vitro technique for assessment of thrombogenicity in mechanical prosthetic cardiac valves: evaluation with a range of valve types Journal of Heart Valve Disease 13(3): 509-520
Chu, Y.-p.; Cheng, J.-l.; Chen, R.-k.; Fan, Y.-b.; Pu, F. 2005: Influence of mimic cardiac rate on hydrodynamics of different mechanical prosthetic cardiac valves in vitro Chinese Medical Sciences Journal 20(2): 138-141
Dalby, A.J.; Stevens, J.E.; Beck, W. 1980: The clinical assessment and management of patients with prosthetic cardiac valves: a review of current practice at the Cardiac Clinic, Groote Schuur Hospital South African Medical Journal 57(9): 307-312
Liu, P.I.; Poon, K.C.; Hong, C.C.; Crook, L.; Liu, S.S.; Eguchi, M. 1980: In vitro and in vivo assessment of the viability of cryopreserved postmortem murine bone marrow cells Annals of Clinical and Laboratory Science 10(2): 165-168
El'bert, L.B.; Vorovich, M.F.; Timofeev, A.V. 1998: Comparative analysis of tests on in vitro and in vivo quantitative assessment of the immunogenicity of tick-borne encephalitis vaccine Voprosy Virusologii 43(5): 236-238
Martin, A.J.; Christy, J.R.E. 2004: Evaluation of an in-vitro thrombosis assessment procedure by application to the Medtronic Parallel and St. Jude Medical valves Journal of Heart Valve Disease 13(4): 667-675
Fraser, J.R.; Clarris, B.J. 1970: On the reactions of human synovial cells exposed to homologous leucocytes in vitro Clinical and Experimental Immunology 6(2): 211-225
O'Brien, M.F. 1972: Comparative analysis of autologous, homologous and heterologous valves Medical Journal of Australia 2(1 Suppl): 51
Gonzalez-Lavin, L.; al-Janabi, N.; Lockey, E.; Ross, D.N. 1973: Fibroblast viability in antibiotic treated valves New Zealand Medical Journal 77(488): 36-39
Buckley, R.H.; Schiff, R.I.; Amos, D.B. 1972: Blocking of autologous and homologous leukocyte responses by human alloimmune plasmas: a possible in vitro correlate of enhancement Journal of Immunology 108(1): 34-44
Shetty, S.; Kaushik, S.; Mojamdar, M.; Gogate, A.; Chaukar, A.P. 1996: Viability testing of homograft valves using methyl thiazol tetrazolium assay Journal of Postgraduate Medicine 42(3): 72-75
Winczyk, K.; Kunert-Radek, J.; Gruszka, A.; Radek, M.; Ławnicka, H.; Pawlikowski, M. 2009: Effects of rosiglitazone--peroxisome proliferators-activated receptor gamma (PPARgamma) agonist on cell viability of human pituitary adenomas in vitro Neuro Endocrinology Letters 30(1): 107-110
Gaudreault, G.; Dumesnil, J.G.; Leblanc, M.H. 1992: Measurement of cardiac output by Doppler echocardiography at the 4 cardiac valves Annales de Cardiologie et d'Angeiologie 41(5): 267-271
Dainese, L.; Barili, F.; Andreini, D.; Guarino, A.; Micheli, B.; Borsetti, C.A.; Polvani, G.; Parolari, A.; Fusari, M.; Biglioli, P. 2008: Cardiac engineered valves: state of the art Giornale Italiano di Cardiologia 9(3): 167-172
Pilichowski, P.; Barrie, J.; Latreille, R. 1980: Flow through various cardiac valves prostheses Journal de Chirurgie 117(2): 129-134
Lim, S.M.; Heng, K.K.; Lim, N.K.; Seah, M.L.; Wee, A.; Li, S.Q.; Soh, P.; Rauff, A.; Vengadasalam, D. 1991: An in vitro assessment of human fetal pancreatic islets of Langerhans in culture Annals of the Academy of Medicine Singapore 20(4): 465-471
Miller, G.L. 1998: Viability assessment before CABG Circulation 98(13): 1351-1352
Boĭkov, P.Ia.; Gumanov, L.L. 1970: Association of some early phage T4B proteins with homologous DNA in vitro Biokhimiia 35(3): 477-483