Bacteriolytic enzyme preparation lysoamidase. Purification and some properties of bacteriolytic peptidase L1
Stepanaia, O.A.; Begunova, E.A.; Tsfasman, I.M.; Kulaev, I.S.
Biokhimiia 61(4): 656-663
1996
ISSN/ISBN: 0320-9725 PMID: 8724784 Document Number: 465442
The bacteriolytic peptidase L1 has been isolated from the enzyme preparation of lysoamidase capable to lyze cell walls of gram-positive bacteria using ion-exchange chromatography and gel filtration. Some physico-chemical properties of the enzyme have been established. The molecular mass of L1 is 21 kDa, the pH optimum for Staphylococcus aureus cell lysis is 7-11. The optimal concentration of the buffer is 50 mM; the temperature optimum is 70 degrees C; the half-inactivation temperature is 55 degrees C.