Skin carcinogenesis induced be neonatal administration of 5-bromo-2'-deoxyuridine and subsequent treatment with 12-O-tetradecanoylphorbol-13-acetate in mice
Anisimov, V.N.; Sheiko, E.V.
Voprosy Onkologii 42(1): 82-84
1996
ISSN/ISBN: 0507-3758 PMID: 8686251 Document Number: 463510
Male and female SHR mice were injected, subcutaneously, 1 mg of 5-bromo-2'-deoxyuridine (BrdUrd) or 0.05 ml of distilled water on days 1, 3 and 7 after birth and painted with 6.15 mkg of TPA dissolved in 0.1 ml of acetone, or acetone alone, twice a week, for 24 weeks, starting at the age of 15 weeks. The animals were followed up for 20 weeks after stopping TPA painting. Skin tumors emerged in TPA-treated animals only. TPA painting was followed by skin papilloma development in 20.9% of males and 36.6% females injected distilled water neonatally, tumors regressing in 88.4% of males and 85.4% of females by the end of the experiment. With combined treatments with BrdUrd and TPA, skin papilloma incidence was 56.5% in males and 52.6% in females, tumors persisting in 52.2% and 39.6%, respectively, until the end of the experiment.