Factors conditioning detection of Taenia saginata antigens in faeces
Machnicka, B.; Dziemian, E.; Zwierz, C.
Applied Parasitology 37(2): 99-105
1996
ISSN/ISBN: 0943-0938 PMID: 8688866 Document Number: 456975
The conditions which optimize detection of T. saginata antigens in faeces were subject of examination. The most appropriate appeared diluent of PBS + 0.3% Tween 20. The highest effectiveness was achieved using capturing and detecting antibodies to surface antigens in comparison with somatic and metabolic ones. Two step centrifugation and final dilution of samples 1:10 (w:v) were recommended. The stability of freezed faecal supernatants after the first centrifugation was limited to 2 months. The sensitivity of capture ELISA established in our examinations allow to signalize 1 ng/ml antigen in high absorbance values. The antigens recognized by the test had MW bigger than 100 000.