Observations of cellular differentiation behaviour in adenoid cystic carcinoma of human salivary gland in vitro
He, R.; Zhou, X.; Xia, W.
Zhonghua Kou Qiang Yi Xue Za Zhi 31(3): 150-152
1996
ISSN/ISBN: 1002-0098 PMID: 9387555 Document Number: 456717
The celluar differentiation behaviour of adenoid cystic carcinoma (ACC) of salivary gland was investigated in vitro with immunohistochemistry. The results showed that the cells of ACC-2 and ACC-3 cell lines can express some marker proteins in vitro including S-100 protein, actin, myosin which is characterized as a feature of myoepithelial cells. When differentiation inducer, dB-cAMP, was added to the monolayer cells cultured in dishes, the marker protein expression increased and the CEA changed to positive. The cellular differetiation behaviour of ACC cells is related to the proliferate speed of cells. Some cells manifested finger ring-like when the culture condition was changed, it means that these cells have the secretory function. If the ACC cells were suspended singly in collagen gels which performed as an extracellular matrix they grew and formed the tubule-like structure. The results will help us to understand the histogenesis of ACC, the source of myoepithelial cells in ACC and the variety of histological structures of ACC.