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Detection of polyphosphatase activity in isolated Saccharomyces cerevisiae nuclei

Lichko, L.P.; Kulakovskaia, T.V.; Dmitriev, V.V.; Kulaev, I.S.

Biokhimiia 60(11): 1911-1916

1995

ISSN/ISBN: 0320-9725
PMID: 8590761
Document Number: 453781
Intact nuclei have been isolated from cells of a diploid strain of Saccharomyces cerevisiae. The isolated nuclei were free from cytoplasmic, mitochondrial and vacuolar marker enzymes. The protein to DNA ratio (w/w) was 11. Pyrophosphatase, tripolyphosphatase and exopolyphosphatase activities have been found in S. cerevisiae nuclei for the first time and were equal to 400, 130 and 55 mU/mg of protein, respectively.

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