Homozygous deletions of the CDKN2 (MTS1/p16ink4) gene in cell lines established from children with acute lymphoblastic leukemia
Zhou, M.; Gu, L.; James, C.D.; He, J.; Yeager, A.M.; Smith, S.D.; Findley, H.W.
Leukemia 9(7): 1159-1161
1995
ISSN/ISBN: 0887-6924 PMID: 7630190 Document Number: 451676
Homozygous deletions of the CDKN2 (MTS1/p16-ink4) gene have been found at high frequency in cell lines derived from a variety of adult solid tumors. In order to investigate the status of the CDKN2 gene in cell lines established from childhood acute lymphoblastic leukemia (ALL), we surveyed 25 lines representing the major pediatric ALL phenotypes for the presence of this gene by Southern blot analysis. Homozygous deletions of all or part of the CDKN2 gene were detected in 21 (84%) cell lines, including 11 of 14 (79%) early-pre-B-ALL, four of five (80%) preB-ALL, and six of six T-ALL lines. CDKN2 mRNA was detected intact CDKN2 gene. These data suggest an important role for by Northern blotting in each of the four lines containing an CDKN2 deletion in the cause and/or progression of pediatric ALL.