Human satellite cell proliferation in vitro is regulated by autocrine secretion of IL-6 stimulated by a soluble factor (s) released by activated monocytes
Cantini, M.; Massimino, M.L.; Rapizzi, E.; Rossini, K.; Catani, C.; Dalla Libera, L.; Carraro, U.
Biochemical and Biophysical Research Communications 216(1): 49-53
1995
ISSN/ISBN: 0006-291X PMID: 7488123 Document Number: 450160
We previously showed that macrophages, besides their scavenger role, selectively induce rat myoblast proliferation in vitro by releasing soluble factors. In this paper we demonstrate a relationship between human-activated monocytes and increased human myoblast proliferation due to IL-6 autocrine secretion by satellite cells. Indeed in the supernatants of muscle cultures treated with activated monocyte-conditioned medium we show by means of an ELISA quantitation a higher autocrine secretion of IL-6 associated with increased myoblast proliferation. This suggests that a growth factor(s) secreted by activated monocytes stimulates IL-6 production by myoblasts and then regulates proliferation of satellite cells.