AMLR and MLR stimulating activity of human T lymphocytes activated in vitro by soluble HLA-DR antigens
Vanoli, M.; Scorza Smeraldi, R.; Fabio, G.; Bonara, P.; Sabbadini, M.G.; Zanussi, C.
Clinical and Experimental Immunology 63(1): 163-170
1986
ISSN/ISBN: 0009-9104 PMID: 2420496 Document Number: 277326
We have examined the allogeneic mixed lymphocyte reaction (MLR) and autologous mixed lymphocyte reaction (AMLR) stimulating activities of T cells precultured in vitro with soluble allogeneic or autologous HLA-DR antigens. These cells (Ts) are known to suppress the human MLR: this suppression is specific in that it occurs only when stimulator cells have the same HLA-DR antigen as that used to induce differentiation of suppressor cells. Ts cells express new membrane specificities; they can be separated by immunoabsorption into two populations: Ts enriched (Tx+; with suppressive activity) and Ts depleted (Ts-; with helper function). In the present study, we have demonstrated that both Ts cell subsets activated by soluble HLA-DR alloantigens are able to stimulate both MLR and AMLR. Ts cells activated by soluble autologous HLA-DR antigens are able to stimulate MLR, but not AMLR.