Quantitative determination of the infective activity of the human immunodeficiency virus by plaque formation using monolayer cultures
Nosik, M.N.; Stepanova, L.G.; Matsevich, G.R.
Voprosy Virusologii 40(5): 202-205
1995
ISSN/ISBN: 0507-4088 PMID: 8659172 Document Number: 448799
A method for assessment of the infective activity of HIV using plaque formation method with human diploid cells (strains L-65, L-68, L-72) has been developed. This method is not inferior to plaque formation with poly-L-lysine in sensitivity, but superior to it in reproducibility of results and standardization of the test conditions. The method is fit for titration of HIV and virus-neutralizing antibodies, as well as for assessment of the activity of anti-HIV preparations.
Document emailed within 1 workday
Related Documents
Hilwig, I.; Schuster, S. 1970: Monolayer cultures of pancreatic tissue. II. Formation of histotypic aggregates from cell suspensions of fetal human pancreas Zeitschrift für Zellforschung und mikroskopische Anatomie 103(2): 282-290Strovel, J.W.; Abruzzo, L.V.; Highsmith, W.E.; Stamberg, J. 1998: Quantitative measurement of telomerase activity in lymphadenopathy: correlation with histologic features and human immunodeficiency virus-1 infection Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology Inc 11(10): 957-962
Kline, M.W.; Lewis, D.E.; Hollinger, F.B.; Reuben, J.M.; Hanson, L.C.; Kozinetz, C.A.; Dimitrov, D.H.; Rosenblatt, H.M.; Shearer, W.T. 1994: A comparative study of human immunodeficiency virus culture, polymerase chain reaction and anti-human immunodeficiency virus immunoglobulin A antibody detection in the diagnosis during early infancy of vertically acquired human immunodeficiency virus infection Pediatric Infectious Disease Journal 13(2): 90-94
Koyanagi, A.; Humphrey, J.H.; Ntozini, R.; Nathoo, K.; Moulton, L.H.; Iliff, P.; Mutasa, K.; Ruff, A.; Ward, B.; Chidawanyika, H.; Hargrove, J.; Mahomva, A.I.; Majo, F.; Malaba, L.C.; Mbizvo, M.T.; Mzengeza, F.; Ndhlovu, M.; Piwoz, E.; Propper, L.; Rambanepasi, P.; Ruff, A.J.; Tavengwa, N.; Zijenah, L.S.; Zunguza, C.D.; Zvandasara, P. 2011: Morbidity among human immunodeficiency virus-exposed but uninfected, human immunodeficiency virus-infected, and human immunodeficiency virus-unexposed infants in Zimbabwe before availability of highly active antiretroviral therapy Pediatric Infectious Disease Journal 30(1): 45-51
Srinivasan, A.; York, D.; Anand, R.; Goldsmith, C.S.; Bohan, C.; Kalyanaraman, V.S. 1987: Rodent and primate monolayer cells support the expression and assembly of viral particles directed by human immunodeficiency virus (HIV) proviral DNA Microbios 50(203): 81-90
Frappa, J.; Trotemann, P.; Beaudry, Y.; Fontanges, R. 1981: Production of IFN beta with human fibroblasts in monolayer cultures. Comparative studies of induction with virus (NDV) and synthetic polynucleotides (poly i - poly C) Developments in Biological Standardization 50: 185-192
Corti, M.E.; Palmieri, O.J.; Villafañe, M.F.; Trione, N. 2004: Evaluation of 61 episodes of infective endocarditis in intravenous drug abusers and human immunodeficiency type-1 virus infection Revista Argentina de Microbiologia 36(2): 85-87
Kuznetsov, S.A.; Luriia, E.A.; Genkina, E.N. 1981: Formation of foci of myeloid cells on colonies of thymus and bone marrow fibroblasts in monolayer cultures Biulleten' Eksperimental'noi Biologii i Meditsiny 91(5): 610-613
Arzumanov, A.A.; Diatkina, N.B.; Kukhanova, M.K.; Kraevskiĭ, A.A.; Semchenko, F.M.; Eremin, O.G.; Martynov, B.I. 1993: 3'-Fluoro-deoxythymidine-5-fluorophosphate--an effective inhibitor of reproduction of human immunodeficiency virus in cell cultures Bioorganicheskaia Khimiia 19(10): 978-980
Shimaniuk, N.I.; Mishan'kin, B.N. 1976: Method for the quantitative determination of penicillin acylase activity by the formation of phenylacetic acid Antibiotiki 21(8): 694-697
Hoffmann, D.; Rathkamp, G.; Nesnow, S.; Wynder, E.L. 1972: Fluoranthenes: quantitative determination in cigarette smoke, formation by pyrolysis, and tumor-initiating activity Journal of the National Cancer Institute 49(4): 1165-1175
Wakabayashi, Y.; Ishiyama, T.; Sugimoto, M.; Abe, S.; Horie, S.; Matsumoto, T.; Hirose, S. 1989: T-cell colony formation in homosexual men with anti-human immunodeficiency virus Nihon Ketsueki Gakkai Zasshi: Journal of Japan Haematological Society 52(1): 80-85
Frantz, J.M.; Murenzi, A. 2013: The physical activity levels among people living with human immunodeficiency virus/acquired immunodeficiency syndrome receiving high active antiretroviral therapy in Rwanda Sahara J: Journal of Social Aspects of Hiv/Aids Research Alliance 10(3-4): 113-118
Sabidó, M.; Lahuerta, M.; Montoliu, A.; Gonzalez, V.; Hernández, G.; Giardina, F.; Monzón, J.é E.; Pedroza, M.I.; Casabona, J. 2011: Human immunodeficiency virus, sexually transmitted infections, and risk behaviors among clients of sex workers in Guatemala: are they a bridge in human immunodeficiency virus transmission? Sexually Transmitted Diseases 38(8): 735-742
Rasheed, S.; Li, Z.; Xu, D.; Kovacs, A. 1996: Presence of cell-free human immunodeficiency virus in cervicovaginal secretions is independent of viral load in the blood of human immunodeficiency virus-infected women American Journal of Obstetrics and Gynecology 175(1): 122-129
Yusim, K.; Peeters, M.; Pybus, O.G.; Bhattacharya, T.; Delaporte, E.; Mulanga, C.; Muldoon, M.; Theiler, J.; Korber, B. 2001: Using human immunodeficiency virus type 1 sequences to infer historical features of the acquired immune deficiency syndrome epidemic and human immunodeficiency virus evolution Philosophical Transactions of the Royal Society of London. Series B Biological Sciences 356(1410): 855-866
Wen, Y.; Guan, J.; Wu, Z.; Li, L.; Rotheram-Borus, M.Jane.; Lin, C.; Detels, R. 2009: Pharmacy workers' sexually transmitted diseases/human immunodeficiency virus knowledge in Fuzhou, China: implications for human immunodeficiency virus testing, treatment, and prevention strategies Sexually Transmitted Diseases 36(4): 221-226
Anonymous 1988: 1988 agent summary statement for human immunodeficiency virus and report on laboratory-acquired infection with human immunodeficiency virus Morbidity and Mortality Weekly Report 37(S-4): 22
Franke, M.F.; Stulac, S.N.; Rugira, I.H.; Rich, M.L.; Bucyibaruta, J.B.; Drobac, P.C.; Iyamungu, G.; Bryant, C.M.; Binagwaho, A.; Farmer, P.E.; Mukherjee, J.S. 2011: High human immunodeficiency virus-free survival of infants born to human immunodeficiency virus-positive mothers in an integrated program to decrease child mortality in rural Rwanda Pediatric Infectious Disease Journal 30(7): 614-616
Baĭsogolov, G.D.; Rudakova, S.F.; Rudakov, L.A.; Konopliannikov, A.G. 1972: Effect of vinblastine on colony formation in monolayer cultures of guinea pig bone marrow and on hematopoiesis in guinea pigs Biulleten' Eksperimental'noi Biologii i Meditsiny 73(9): 95-98