Radiation-induced irreversible g (0) g (1) block is abolished in human-diploid fibroblasts transfected with the human papilloma-virus e6 gene - implication of the p53-cip1 waf1 pathway
Li, C.; Nagasawa, H.; Tsang, N.; Little, J.
International Journal of Oncology 6(1): 233-236
1995
ISSN/ISBN: 1019-6439 PMID: 21556528 Document Number: 447679
It has been shown previously with human diploid fibroblasts that a fraction of cells remains irreversibly blocked in the G(0)/G(1) phase of the cell cycle when the cells are irradiated in confluent, density-inhibited cultures and released by subculture to low density. In the present study we demonstrate that this G(0)/G(1) block is abolished when a human fibroblast cell strain is transfected with the human papilloma virus 16 E6 gene. Corresponding to the abolition of the G(0)/G(1) block in the E6-transfected cells, the expression of both the p53 and WAF1 genes is also abolished, suggesting that the p53 pathway of DNA damage response may be involved in this phenomenon.