Preliminary results on the use of human diploid fibroblasts for inactivated polio virus vaccine production
Larsson, B.; Litwin, J.
Developments in Biological Standardization 46: 241-247
1980
ISSN/ISBN: 0301-5149 PMID: 6245000 Document Number: 156857
Human diploid fibroblast strains WI-38 and MRC 5 can be routinely grown in Eagles medium to densities between 2x10(5) and 1x10(5) cells/cm2. Polio virus type 1 grew to higher titers in WI-38 than MRC 5; types 2 and 3 grew equally well in both strains. After inactivation with 5 mM formaldehyde at 30 degrees C immunogenicity values were obtained which compared favourably with those obtained from monkey kidney preparations.