Protection of myocardial tissue against deleterious effects of oxygen free radicals by ceruloplasmin
Mateescu, M.A.; Chahine, R.; Roger, S.; Atanasiu, R.; Yamaguchi, N.; Lalumière, G.; Nadeau, R.
Arzneimittel-Forschung 45(4): 476-480
1995
ISSN/ISBN: 0004-4172 PMID: 7779145 Document Number: 445554
This study describes the cardioprotective effect of ceruloplasmin (CAS 9031-37-2) against oxygen free radical injury, as indicated by several biochemical indicators and some cardiodynamic variables. Isolated rat hearts (n = 4-8, p lt 0.05, for each experimental point) in Langendorff preparation were exposed to oxygen free radicals generated by electrolysis (10 mA) in the absence and the presence of 0.25 mu-mol/l purified ceruloplasmin and denaturated ceruloplasmin, in Krebs-Henseleit perfusion solutions. Biochemical indicators (noradrenaline, malondialdehyde, creatine-kinase, lactate dehydrogenase, aspartate aminotransferase, Ca-2+ and Mg-2+) as well as the electrocardiogram and the left ventricular pressure (LVP), were altered by oxygen free radicals formation, denoting major cellular and tissular damages in the nontreated hearts. Ceruloplasmin exhibited a cardioprotective effect and prevented the oxygen free radical-induced release of noradrenaline, indicating that it can also protect the sympathetic nerve endings from oxygen free-radical injury. Purified ceruloplasmin, a circulating extracellular antioxidant and oxygen free radical scavenger seems to be an effective heart protective agent against myocardial and neuronal injuries generated by oxygen free radicals.