A PCR method for examining diarrhea--Escherichia coli
Itoh, F.; Yamaoka, K.; Ogino, T.; Kanbe, M.
Rinsho Byori. Japanese Journal of Clinical Pathology 43(8): 772-775
1995
ISSN/ISBN: 0047-1860 PMID: 7474434 Document Number: 444820
To identify diarrhea--Escherichia coli(E. coli), especially Enteroinvasive E. coli(EIEC), Verocytotoxin-producing E. coli(VTEC), and Enterotoxigenic E. coli(ETEC), confirmation of pathogenic factors is necessary. We developed a system using the polymerase chain reaction(PCR) that simultaneously confirms the pathogenic genes of these E. coli, and have used it in routine examination. This method is simple and does not require examination for each pathogenic factor. Multiple pathogenic genes could be rapidly detected.