Comparative study of chromatographic spectra of polypeptides extracted from pig spleen, liver, kidneys, thymus and periodontium
Kaĭdashev, I.P.
Ukrainskii Biokhimicheskii Zhurnal 67(5): 85-89
1995
ISSN/ISBN: 0201-8470 PMID: 8830442 Document Number: 443748
Polypeptide extracts from the pig spleen, liver, kidney, thymus, pancreas and periodont were obtained by the acid extraction in the presence of Zn and MG cations. These extracts were analyzed by HPLC with determining amino acid sequence using Edman's method. According to the analysis results several fragments of hemoglobin are present in the group of parenchymal organs (epsilon-chain pig hemoglobin 56-66 and 55-66, alpha-chain 48-61), the main mass of substances is localized in more hydrophilic part of chromatogram with 20 amino acid N-terminal ubiquitin fragments; hydrophilic polypeptides ubiquitin and 2 hydrophobic polypeptides were also found in the group, but hemoglobin peaks were absent. The main mass of polypeptide substances in periodont extract is localized in the hydrophobic region, the peaks of hemoglobin and ubiquitin are absent. We support that each group of similar morpho-functional tissues has similar chromatographic picture of polypeptide extracts. It can be connected with the processing of protein structures specific for each organ or tissue.