Alternatively spliced adenomatous polyposis coli (APC) gene transcripts that delete exons mutated in attenuated APC
Samowitz, W.S.; Thliveris, A.; Spirio, L.N.; White, R.
Cancer Research 55(17): 3732-3734
1995
ISSN/ISBN: 0008-5472 PMID: 7641184 Document Number: 441167
Reverse transcription-PCR combined with either (a) restriction enzyme digestion and repeat PCR or (b) ligase chain reaction has identified two new alternatively spliced transcripts of the adenomatous polyposis coli (APC) gene. In one of these transcripts exons 1-4 and the first 16 bases of exon 5 are deleted; in the other exons 2-4 and the first 16 bases of 5 are deleted. Both transcripts use an intraexonic splice acceptor in exon 5. These transcripts delete exons mutated in attenuated APC (3 and 4) and could account for the reduction in severity of this variant.