Morphological change in the retina after injection of calcium and netilmicin sulfate in the rabbit vitreous body
Takeichi, Y.; Miki, K.; Uyama, M.
Nippon Ganka Gakkai Zasshi 98(2): 150-156
1994
ISSN/ISBN: 0029-0203 PMID: 8109459 Document Number: 436048
We reported previously selective damage of the outer segment of the photoreceptors and retinal pigment epithelium after intravitreal injection of netilmicin sulfate in the rabbit. The cellular damage was thought due to active damage of phospholipase C enzyme, preventing it from performing phospholipid inositol metabolism, and to the presence of calcium. Therefore we investigated the retinal damage following after intravitreal injection of calcium and netilmicin sulfate in the rabbit. Morphologically, the retina did not show any structural change after intravitreal injection of CaCl-2 at any concentration. After an intravitreal injection of netilmicin sulfate mixed with CaCl-2, the damage in the photoreceptor outer segment was reduced at a concentration of 200 mu-g/0.1 ml and in the retinal pigment epithelium at a concentration of 1,000 mu-g/ 0.1 ml. This showed that retinal damage due to netilmicin sulfate can be reduced by the addition of calcium.