Matrix-assisted laser desorption mass spectrometric analysis of a pegylated recombinant protein
Watson, E.; Shah, B.; DePrince, R.; Hendren, R.W.; Nelson, R.
Biotechniques 16(2): 278-281
1994
ISSN/ISBN: 0736-6205 PMID: 7514005 Document Number: 435793
Matrix-assisted laser desorption ionization mass spectrometry (MALDI) has been investigated as a technique for the characterization of recombinant stem cell factor that had been covalently modified with polyethylene glycol (PEG) chains. The attachment of PEG chains produces a heterogeneous mixture of protein species that differ by 6000 Da. These differences in molecular weight could be readily determined by MALDI. The potential of MALDI as a general strategy for characterizing PEG-modified proteins is discussed.
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