The cultivation of Vero cells and human embryonic fibroblasts in serum-free media and in media with a low serum content
Nikolaenko, N.S.; Tsupkina, N.V.; Nikitina, I.M.; Pinaev, G.P.
Tsitologiia 36(8): 816-821
1994
ISSN/ISBN: 0041-3771 PMID: 7701612 Document Number: 435645
The optimum composition of serum-free media has been established for cultivation of the continuous cell line Vero originated from kidney tissue of a normal African green monkey, in addition to the optimum composition of low-level serum media (2%) for cultivation of human embryonic fibroblasts. The medium DME (one of the basic media) appeared a medium of choice for both these cell types, and the best serum replacement consisted of insulin, bovine serum albumin, ethanolamine, epidermal growth factor and hydrocortisone. Under employment of this serum replacement together with the medium DME the proliferation indices for the Vero lineage and human embryonic fibroblasts were 76 and 71%, respectively. The developed serum replacement can serve the basis for a further optimization on cultivation of fibroblast-like cells, such as Vero and human embryonic fibroblasts, under serum-free or low-level serum conditions.