A new two-stage plan for isolating human alpha1-acidic glycoprotein (orosomucoid) . Orosomucoid interacts with ethidium bromide
Trubetskaia, O.E.; Muranova, T.A.; Rezikova, O.I.; Afanas'eva, G.V.; Reznikov, K.I.
Bioorganicheskaia Khimiia 20(12): 1297-1309
1994
ISSN/ISBN: 0132-3423 PMID: 7695648 Document Number: 430642
Human alpha 1-acid glycoprotein (orosomucoid, AGP) was purified to homogeneity by a two-step procedure using phenol and chloroform deproteinization of serum with subsequent preparative electrophoresis on agarose gel. It was obtained 0.15-0.3 mg of protein from 1 ml of serum. Human alpha 1-AGP binds ethidium bromide but not DNA. Neither other human serum proteins nor mice and rat alpha 1-AGP bind ethidium bromide. This property of human orosomucoid depends on its amino acid sequence rather than on the carbohydrate part of the molecule.