A new computer analysis technique using automatic threshold selection algorithm, available for quantitative estimation of the immunostained dendrites of the cerebellum
Moriya, M.; Inoue, Y.; Tanaka, S.
Kaibogaku Zasshi. Journal of Anatomy 69(2): 161-167
1994
ISSN/ISBN: 0022-7722 PMID: 7517610 Document Number: 429318
We developed a new computer image analysis technique with application of an automatic threshold selection (ATS) algorithm which was successfully used in the quantitative evaluation of a highly complex texture of immunostained brain tissue. We applied this new method for estimation of the volume of the Purkinje dendrites, which were immunohistochemically stained for inositol 1,4,5-trisphosphate receptor protein (P-400), and demonstrated a significant reduction of the dendritic arborization of the Purkinje cells of a neurological mutant mouse. Wriggle mouse Sagami, which would appear normal if stained with conventional methods such as H-E and Nissle staining.