The mechanism of the immunostimulating action of trimezon
Dad'kov, I.G.; Liubin, G.S.; Kuz'mitskiĭ, B.B.
Eksperimental'naia i Klinicheskaia Farmakologiia 57(2): 45-47
1994
ISSN/ISBN: 0869-2092 PMID: 8205049 Document Number: 429301
The influence of various concentrations (10(-9) - 10(-6) M) of the new immunostimulator trimezon of 8-aza-D-homogonan range upon in vitro proliferation of human lymphocytes, stimulated by PHA 0.5-10.0 micrograms/ml), was investigated by 3H-thymidine incorporation analysis. The maximal stimulating effect of trimezon (by 2.5 times) was found at trimezon concentration of 10(-8) M and at blast transformation optimal PHA concentration of 1 microgram/ml. In the models of the induction of lymphocyte suppressor activity by ConA high concentrations in vitro and by syngeneic mouse hyperimmunized cell transfer in vivo was demonstrated the ability of trimezon to increase the activity of both helper and suppressor sets of regulation mechanisms of immune response. This characterises trimezon as a true immunomodulator.