The presence in Trypanosoma cruzi microsomes of alpha (1,2) , alpha (1,3) and alpha (1,6) mannosidase activities not involved in protein-linked Man9GlcNAc2 processing
Xavier, M.T.; Merello, S.; Parodi, A.J.
Cellular and Molecular Biology 40(7): 989-997
1994
ISSN/ISBN: 0145-5680 PMID: 7849566 Document Number: 427129
Trypanosoma cruzi microsomes were found to possess membrane-bound alpha(1,2), alpha(1,3) and alpha(1,6) mannosidase activities that had an almost neutral optimum pH value, did not require CaCl2 for activity and were inhibited by swainsonine but not by deoxymannojirimycin. A mannosidase activity that degraded p-nitrophenylmannoside and that was inhibited by swainsonine was also present in the parasite microsomes. Experiments performed with intact cells showed that processing of protein-linked Man9GlcNAc2 was inhibited by deoxymannojirimycin but not by swainsonine. It was concluded that the activities detected were not involved in protein-linked Man9GlcNAc2 processing.